DNA

Part:BBa_K2644009:Design

Designed by: Yiran Cheng   Group: iGEM18_TJU_China   (2018-09-30)


dcas9+ω


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
    Illegal BamHI site found at 4212
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Directly PCR from pwj66.


Source

It come from a plasmid named pwj66 on website Addgene.

References